GORE^® Protein Capture Devices with Protein A

Traditional affinity chromatography technology has not kept pace with increased titers and other upstream process improvements, leading to bottlenecks and lower productivity in downstream purification. To gain flow and shorten processing times, traditional resin-based Protein A is often over-sized or underutilized – simply to keep up.*

*Reference: “Disrupting Downstream Process Bottlenecks.” Genetic Engineering, June 14, 2018.

Increase productivity: GORE Protein Capture Devices with Protein A utilize a unique membrane solution that provides a binding capacity advantage at high flow rates and improves the speed of purification versus traditional technologies. Compare our Dynamic Binding Capacity (DBC) to that of agarose bead-based columns:

GORE Protein Capture Devices are approximately 6-10x faster than traditional agarose bead-based technology which operates at typical residence times of 3–5 minutes (180–300 seconds).

Designed for affinity purification in early drug discovery screening, late stage optimization, process development, and clinical applications for

• monoclonal antibodies (mAbs)
• next generation molecules that utilize the FC region for Protein A binding

The columns are compatible with standard chromatography systems.

GORE Protein Capture Devices with immobilized Protein A* can improve throughput and yields in downstream purification by reducing overall process time. These pre-packed columns use a unique ePTFE membrane that offers high dynamic binding capacity at short residence time. The 58 mL - 232 mL accommodate rapid cycle capability at low pressure drop, along with demonstrated ability to withstand multiple cleaning cycles. This increased productivity offers researchers the potential for a faster path to clinical trials.

The GORE Protein Capture Device scales across sizes without compromising binding capacity or speed to quickly and effectively capture antibodies as shown in this overlay of a 1mL, 9mL and 58mL bind-elute curve.

For complete technical and product performance information, including detailed test parameters, additional yield data, purity profiles, operating conditions and more, download our data sheet.

The GORE Protein Capture Device can streamline antibody purification processes because it incorporates a proprietary ePTFE membrane composite.

Unlike traditional support matrices, the composite membrane bed maintains a linear relationship between pressure drop and a wide range of flow rates. Because this unique structure remains stable, the membrane bed is not vulnerable to collapse, channeling or alteration of the bed.

GORE Protein Capture Devices are compatible with standard pre-packed column chromatography systems, and are available in 1.0 ml, 3.5mL, 9.0mL, 58mL, 116 mL and 232 mL sizes. Contact Gore for pricing and availability. Not available in all regions.

GORE Protein Capture Devices are manufactured following the applicable material quality and regulatory requirements, including relevant Good Manufacturing Processes as defined in the Gore PharmBIO quality system which is certified to ISO 13485 and ISO 15378. Contact Gore for current applicable compliance statements and quality control information.