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Image of Application Note for GORE® Protein Capture Device Used in Series

Chromatography

Two 3.5 mL GORE Protein Capture Devices can be connected in series, which doubles the column capacity by providing a 7.0 mL bed volume and allows capture of at least 200 mg of monoclonal antibody within 60 minutes.

English (U.S.)

Image of Application Note for GORE® Protein Capture Device Used in Manual LC Methods

Chromatography

The GORE Protein Capture Device with Protein A can be used as demonstrated in three different manual/semi-automated operations to rapidly purify monoclonal antibodies without an LC unit.

English (U.S.)

Application Note: Aragen's A Platform to Rapidly Purify and Formulate Immunoglobulins from Mammalian Expression Systems

Chromatography

Recently introduced technologies such as Protein A membrane devices and tangential flow filtration (TFF) systems capable of handling ultrafiltration and diafiltration of samples as low as 5 mL have significantly decreased the turnaround time.

This document is available upon request.

English (U.S.)

Increasing the Productivity of mAb Purification using the GORE Protein Capture Devices with Protein A

Chromatography

Evaluate the impact of increasing operational flow rate steps (e.g. equilibrations, washes, and clean-in-place (CIP)) on the overall productivity (mg mAb/hour) of GORE Protein Capture Devices (PROA101 & PROA102).

English (U.S.)

Thumbnail image of Application Note

Chromatography

Boehringer-Ingelheim evaluated GORE Protein Capture Devices with Protein A vs. a traditional agarose bead column and found that, together with high titers, the GORE column can reduce overall process time and may help increase yields in monoclonal antibody purification by eliminating the need for additional downstream concentration steps.

English (U.S.)

Image of Application Note for GORE® Protein Capture Device Cleaning Protocols

Chromatography

Two cleaning protocols outlined in this application note potentially extend the lifetime of a GORE Protein Capture Device in the event that pressure elevates over the expected lifetime of the device.

English (U.S.)

Application Note:  Extending the Lifetime of GORE® Protein Capture Devices

Chromatography

This evaluation demonstrates a method to extend the lifetime of a GORE Protein Capture Device from 20 to 50 cycles while maintaining capacity as assessed by Dynamic Binding Capacity (DBC) at 10% breakthrough, yield, and purity.

English (U.S.)

Application Note:  Increase productivity with a simulated moving bed chromatography technique.

Evaluate the GORE® Protein Capture Device with Protein A combined with a simulated moving bed chromatography technique, CaptureSMB(TM), to increase the productivity of IgG purification. A joint study with Athena Enzyme Systems and Gore & Associates.

English (U.S.)

Application Note: Sodium Hydroxide (NaOH) Exposure Conditions for Successful Operation of GORE<sup>®</sup> Protein Capture Devices with Protein A for Drug Discovery Applications

Chromatography

This application note provides data to support sodium hydroxide (NaOH) exposure limits for the 1.0mL and 3.5mL GORE Protein Capture Devices (PROA101 and PROA102). The cycling protocol utilizing 0.5 molar (M) NaOH supports 50 purification cycles with minimal impact to purification yield, device capacity, or assessed purity.

English (U.S.)

Thumbnail image of Pharmaceutial Processing article reprint

Lyophilization

Article: Lyophilization: The Basics

Industry Articles, 540.88 KB,

Cherish Robinson, Product Specialist with Gore's PharmBIO business, discusses the advantages and disadvantages of freeze-drying bulk drug ingredients.

English (U.S.)