Resource Library

General handling, antibody purification protocol and troubleshooting guide for 58mL (PROA201), 116 mL (PROA202), and 232 mL (PROA203).

This evaluation demonstrates a method to improve productivity by increasing flow rates for the non-loading steps with a representative clarified CHO cell harvest with the 58mL, GORE Protein Capture Device (PROA201) and maintaining critical quality attributes including yield, purity and elution widths.

General handling, antibody purification protocol, troubleshooting, and ordering information.

The GORE Protein Capture Device with Protein A can be used as demonstrated in three different manual/semi-automated operations to rapidly purify monoclonal antibodies without an LC unit.

Two 3.5 mL GORE Protein Capture Devices can be connected in series, which doubles the column capacity by providing a 7.0 mL bed volume and allows capture of at least 200 mg of monoclonal antibody within 60 minutes.

This application note provides data to support sodium hydroxide (NaOH) exposure limits for the 1.0mL and 3.5mL GORE Protein Capture Devices (PROA101 and PROA102). The cycling protocol utilizing 0.5 molar (M) NaOH supports 50 purification cycles with minimal impact to purification yield, device capacity, or assessed purity.

General handling, antibody purification protocol and troubleshooting guide for PROA103.

Evaluate the GORE® Protein Capture Device with Protein A combined with a simulated moving bed chromatography technique, CaptureSMB(TM), to increase the productivity of IgG purification. A joint study with Athena Enzyme Systems and Gore & Associates.

Two cleaning protocols outlined in this application note potentially extend the lifetime of a GORE Protein Capture Device in the event that pressure elevates over the expected lifetime of the device.

This evaluation demonstrates a method to extend the lifetime of a GORE Protein Capture Device from 20 to 50 cycles while maintaining capacity as assessed by Dynamic Binding Capacity (DBC) at 10% breakthrough, yield, and purity.